spatialHeatmap 0.99.0
[ThG-Comment: to-dos are now mostly listed as margin notes on right.]
The spatialHeatmap package provides functionalities for visualizing cell-,
tissue- and organ-specific data of biological assays by coloring the
corresponding spatial features defined in anatomical images according to a
numeric color key. The color scheme used to represent the assay values can be
customized by the user. This core functionality of the package is called a
spatial heatmap (SHM) plot. It is enhanced with nearest neighbor
visualization tools for groups of measured items (e.g. gene modules) sharing
related abundance profiles, including matrix heatmaps combined with
hierarchical clustering dendrograms and network representations. The
functionalities of spatialHeatmap can be used either in a command-driven mode
from within R or a graphical user interface (GUI) provided by a Shiny App that
is also part of this package. While the R-based mode provides flexibility to
customize and automate analysis routines, the Shiny App includes a variety of
convenience features that will appeal to experimentalists and other users less
familiar with R. Moreover, the Shiny App can be used on both local computers as
well as centralized server-based deployments (e.g. cloud-based or custom
servers) that can be accessed remotely as a public web service for using
spatialHeatmap’s functionalities with community and/or private data. The
functionalities of the spatialHeatmap package are illustrated in Figure
1.
Figure 1: Overview of spatialHeatmap
(A) The saptialHeatmap package supports assay data from a wide range of omics technologies including genomic, transcriptomic, proteomic and metabolomic profiling data. The assay data can be provided as numeric vectors, tabular data, or SummarizedExperiment objects. The latter is a widely used data container for organizing both assay data as well as associated annotation and experimental design data. (B) Anatomical and other spatial images are provided as annotated SVG (aSVG) files where the spatial features and the corresponding data components of the assay data have matching labels (e.g. tissue labels). (C) The assay data are used to color the matching spatial features in one or more aSVG images according to a color key. The result is called a spatial heatmap (SHM) plot. Multiple measurements can be visualized in the same plot, such as several factors (e.g. genes, proteins, metabolites), treatment conditions, growth stages and more. (D) Data mining graphics, such as matrix heatmaps and network graphs, are integrated to facilitate the identification of factors with similar assay profiles. The functionalities of spatialHeatmap can be accessed from local computers via the R console or a graphical user interface based on Shiny. In addtion, the latter can be deployed as a web service on custom servers or cloud-based systems.
As anatomical images the package supports both tissue maps from public repositories and custom images provided by the user. In general any type of image can be used as long as it can be provided in SVG (Scalable Vector Graphics) format, where the corresponding spatial features have been defined (see aSVG below). The numeric values plotted onto an SHM are usually quantitative measurements from a wide range of profiling technologies, such as microarrays, next generation sequencing (e.g. RNA-Seq and scRNA-Seq), proteomics, metabolomics, or many other small- or large-scale experiments. For convenience, several preprocessing and normalization methods for the most common use cases are included that support raw and/or preprocessed data. Currently, the main application domains of the spatialHeatmap package are numeric data sets and spatially mapped images from biological, agricultural and biomedical areas. Moreover, the package has been designed to also work with many other spatial data types, such a population data plotted onto geographic maps. This high level of flexibility is one of the unique features of spatialHeatmap. Related software tools for biological applications in this field are largely based on pure web applications (Lekschas et al. 2015; Papatheodorou et al. 2018; Winter et al. 2007; Waese et al. 2017) or local tools (Maag 2018; Muschelli, Sweeney, and Crainiceanu 2014) that typically lack customization functionalities. These restrictions limit users to utilizing pre-existing expression data and/or fixed sets of anatomical image collections. To close this gap for biological use cases, we have developed spatialHeatmap as a generic R/Bioconductor package for plotting quantitative values onto any type of spatially mapped images in a programmable environment and/or in an intuitive to use GUI application.
The core feature of spatialHeatmap is to map the assay values (e.g.
gene expression data) of one or many items (e.g. genes) measured under
different conditions in form of numerically graded colors onto the
corresponding cell types or tissues represented in a chosen SVG image. In the
gene profiling field, this feature supports comparisons of the expression
values among multiple genes by plotting their SHMs next to each
other. Similarly, one can display the expression values of a single or multiple
genes across multiple conditions in the same plot (Figure 3). This level of flexibility is
very efficient for visualizing complicated expression patterns across genes,
cell types and conditions. In case of more complex anatomical images composed
of overlapping multiple layer tissues, it is important to visually expose the
tissue layer of interest in the plots. To address this, several default and
customizable layer viewing options are provided. They allow to hide features in
the top layers by making them transparent in order to expose features below
them. This transparency viewing feature is highlighted below in the mouse
example (Figure 6). Moreover, one can plot multiple distinct
aSVGs in a single SHM plot as shown in Figure 9. This is
particularly useful for displaying abundance trends across multiple development
stages, where each is represented by its own aSVG image. In addition to
static SHM representations, one can visualize them in form of dynamic animations
that are supported a rich set of interactive functionalities, such as zooming and video download
options.
To maximize reusability and extensibility, the package organizes large-scale
omics assay data along with the associated experimental design information in a
SummarizedExperiment object (Figure 1A). The latter is one of the core S4 classes within
the Bioconductor ecosystem that has been widely adapted by many other software
packages dealing with gene-, protein- and metabolite-level profiling data
(Morgan et al. 2018). In case of gene expression data, the assays slot of
the SummarizedExperiment container is populated with a gene expression
matrix, where the rows and columns represent the genes and tissue/conditions,
respectively, while the colData slot contains sample data including replicate
information. The tissues and/or cell type information in the object maps via
colData to the corresponding features in the SVG images using unique
identifiers for the spatial features (e.g. tissues or cell types). This
allows to color the features of interest in an SVG image according to the
numeric data stored in a SummarizedExperiment object. For simplicity the
numeric data can also be provided as numeric vectors or data.frames. This
can be useful for testing purposes and/or the usage of simple data sets that
may not require the more advanced features of the SummarizedExperiment class,
such as measurements with only one or a few data points. The details about how to
access the SVG images and properly format the associated expression data are
provided in the Supplementary Section of this vignette.
SHMs are images where colors encode numeric values in features of any shape. For plotting SHMs, Scalable Vector Graphics (SVG) has been chosen as image format since it is a flexible and widely adapted vector graphics format that provides many advantages for computationally embedding numerical and other information in images. SVG is based on XML formatted text describing all components present in images, including lines, shapes and colors. In case of biological images suitable for SHMs, the shapes often represent anatomical or cell structures. To assign colors to specific features in SHMs, annotated SVG (aSVG) files are used where the shapes of interest are labeled according to certain conventions so that they can be addressed and colored programmatically. SVGs and aSVGs of anatomical structures can be downloaded from many sources including the repositories described below. Alternatively, users can generate them themselves with vector graphics software such as Inkscape. Typically, in aSVGs one or more shapes of a feature of interest, such as the cell shapes of an organ, are grouped together by a common feature identifier. Via these group identifiers one or many feature types can be colored simultaneously in an aSVG according to biological experiments assaying the corresponding feature types with the required spatial resolution. Correct assignment of image features and assay results is assured by using for both the same feature identifiers. The color gradient used to visually represent the numeric assay values is controlled by a color gradient parameter. To visually interpret the meaning of the colors, the corresponding color key is included in the SHM plots. Additional details for properly formatting and annotating both aSVG images and assay data are provided in the Supplementary Section section of this vignette.
If not generated by the user, SHMs can be generated with data downloaded from
various public repositories. This includes gene, protein and metabolic
profiling data from databases, such as GEO,
BAR and Expression
Atlas from EMBL-EBI (Papatheodorou et al. 2018). A
particularly useful resource, when working with spatialHeatmap, is the EBI
Expression Atlas. This online service contains both assay and anatomical
images. Its assay data include mRNA and protein profiling experiments for
different species, tissues and conditions. The corresponding anatomical image
collections are also provided for a wide range of species including animals and
plants. In spatialHeatmap several import functions are provided to work with
the expression and aSVG repository from the Expression Atlas
directly. The aSVG images developed by the spatialHeatmap project are
available in its own repository called spatialHeatmap aSVG
Repository,
where users can contribute their aSVG images that are formatted according to
our guidlines.
The following sections of this vignette showcase the most important
functionalities of the spatialHeatmap package using as initial example a simple
to understand toy data set, and then more complex mRNA profiling data from the
Expression Atlas and GEO databases. First, SHM plots are generated for both the toy
and mRNA expression data. The latter include gene expression data sets from
RNA-Seq and microarray experiments of Human Brain, Mouse
Organs, Chicken Organs, and Arabidopsis Shoots. The
first three are RNA-Seq data from the Expression
Atlas, while the last one is a microarray data
set from GEO. Second, gene context
analysis tools are introduced, which facilitate the visualization of
gene modules sharing similar expression patterns. This includes the
visualization of hierarchical clustering results with traditional matrix
heatmaps (Matrix Heatmap) as well co-expression network plots
(Network). Third, an overview of the corresponding Shiny App
is presented that provides access to the same functionalities as the R
functions, but executes them in an interactive GUI environment (Chang et al., n.d.; Chang and Borges Ribeiro 2018). Fourth, more advanced features for plotting customized
SHMs are covered using the Human Brain data set as an example.
The spatialHeatmap package should be installed from an R (version \(\ge\) 3.6)
session with the BiocManager::install command.
if (!requireNamespace("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("spatialHeatmap")
Next, the packages required for running the sample code in this vignette need to be loaded.
library(spatialHeatmap); library(SummarizedExperiment); library(ExpressionAtlas); library(GEOquery)
The following lists the vignette(s) of this package in an HTML browser. Clicking the corresponding name will open this vignette.
browseVignettes('spatialHeatmap')
SHMs are plotted with the spatial_hm function. To provide a quick
and intuitive overview how these plots are generated, the following uses a
generalized toy example where a small vector of random numeric values is
generated that are used to color features in an aSVG image. The image chosen
for this example is an aSVG depicting the human brain. The corresponding image
file ‘homo_sapiens.brain.svg’ is included in this package for testing purposes.
The path to this image on a user's system, where spatialHeatmap is
installed, can be obtained with the system.file function.
The following commands obtain the directory of the aSVG collection and the full path to the chosen target aSVG image on a user’s system, respectively.
svg.dir <- system.file("extdata/shinyApp/example", package="spatialHeatmap")
svg.hum <- system.file("extdata/shinyApp/example", 'homo_sapiens.brain.svg', package="spatialHeatmap")
To identify feature labels of interest in annotated aSVG images, the return_feature
function can be used. The following searches the aSVG images stored in dir
for the query terms ‘lobe’ and ‘homo sapiens’ under the feature and species
fields, respectively. The identified matches are returned as a data.frame.
feature.df <- return_feature(feature=c('lobe'), species=c('homo sapiens'), remote=FALSE, dir=svg.dir)
## Accessing features...
## arabidopsis_thaliana.organ_shm.svg, arabidopsis_thaliana.organ_shm1.svg, arabidopsis_thaliana.organ_shm2.svg, arabidopsis_thaliana.root.cross_shm.svg, arabidopsis_thaliana.root.ebi_shm.svg, arabidopsis_thaliana.root.roottip_shm.svg, arabidopsis_thaliana.shoot_shm.svg, arabidopsis_thaliana.shoot.root_shm.svg, gallus_gallus.svg, homo_sapiens.brain.svg, mus_musculus.male.svg, us_map_shm.svg,
feature.df
## feature id SVG parent index index1
## 1 occipital lobe UBERON_0002021 homo_sapiens.brain.svg LAYER_EFO 9 7
## 2 parietal lobe UBERON_0001872 homo_sapiens.brain.svg LAYER_EFO 10 8
## 3 temporal lobe UBERON_0001871 homo_sapiens.brain.svg LAYER_EFO 26 24
fnames <- feature.df[, 1]
The following example generates a small numeric toy vector, where the data slot
contains four numbers and its name slot is populated with the three feature
names obtained from the above aSVG image. In addition, a non-matching entry
(here ‘notMapped’) is included for demonstration purposes. Note, the numbers
are mapped to features via matching names among the numeric vector and the aSVG,
respectively. Accordingly, only numbers and features with matching name
counterparts can be colored in the aSVG image. Entries without name matches
are indicated by a message printed to the R console, here “notMapped”. This
behavior can be turned off with verbose=FALSE in the corresponding function
call. In addition, a summary of the numeric assay to feature mappings is stored
in the result data.frame returned by the spatial_hm function (see below).
my_vec <- sample(1:100, length(unique(fnames))+1)
names(my_vec) <- c(unique(fnames), 'notMapped')
my_vec
## occipital lobe parietal lobe temporal lobe notMapped
## 92 77 16 97
Next, the SHM is plotted with the spatial_hm function (Figure
2). Internally, the numbers in my_vec are translated into
colors based on the color key assigned to the col.com argument, and then
painted onto the corresponding features in the aSVG, where the path to the image
file is defined by svg.path=svg.hum. The remaining arguments used here include:
ID for defining the title of the plot; ncol for setting the column-wise layout
of the plot excluding the feature legend plot on the right; and height for defining
the height of the SHM relative to its width. In the given example
(Figure 2) only three features in my_vec (‘occipital lobe’,
‘parietal lobe’, and ‘temporal lobe’) have matching entries in the corresponding
aSVG.
shm.df <- spatial_hm(svg.path=svg.hum, data=my_vec, ID='toy', ncol=1, height=0.9, width=0.8, sub.title.size=20, legend.nrow=2)
## Syntactically valid column names are made!
## Coordinates: homo_sapiens.brain.svg ...
## Enrties not mapped: notMapped
## Grobs: homo_sapiens.brain.svg ...
## SHMs and legend...
Figure 2: SHM of human brain with toy data
The plots from left to right represent: color key, SHM and legend. The colors in the first two plots depict the user provided numeric values, whereas in the legend plot they are used to map the feature labels to the corresponding spatial regions in the image.
The named numeric values in my_vec, that have name matches with the features in the
chosen aSVG, are stored in the mapped_feature slot.
# The SHM and mapped features are stored in a list
names(shm.df)
## [1] "spatial_heatmap" "mapped_feature"
# Mapped features
shm.df[['mapped_feature']]
## rowID featureSVG value SVG
## 1 toy occipital.lobe 92 homo_sapiens.brain.svg
## 2 toy parietal.lobe 77 homo_sapiens.brain.svg
## 3 toy temporal.lobe 16 homo_sapiens.brain.svg
This subsection introduces how to find cell- and tissue-specific assay data in
the Expression Atlas database. After choosing a gene expression experiment, the
data is downloaded directly into a user's R session. Subsequently, the
expression values for selected genes can be plotted onto a chosen aSVG image with
or without prior preprocessing steps (e.g. normalization). For querying and
downloading expression data from the Expression Atlas database, functions from
the ExpressionAtlas package are used (Keays 2019).
The following example searches the Expression Atlas for expression data derived from specific tissues and species of interest, here ‘cerebellum’ and ‘Homo sapiens’, respectively.
all.hum <- searchAtlasExperiments(properties="cerebellum", species="Homo sapiens")
The search result is stored in a DFrame containing
accessions matching the above query. For the following sample code, the
accession
‘E-GEOD-67196’
from Prudencio et al. (2015) has been chosen, which corresponds
to an RNA-Seq profiling experiment of ‘cerebellum’ and ‘frontal cortex’ brain
tissue from patients with amyotrophic lateral sclerosis (ALS). Details about the
corresponding record can be returned as follows.
all.hum[2, ]
## DataFrame with 1 row and 4 columns
## Accession Species Type Title
## <character> <character> <character> <character>
## 1 E-GEOD-67196 Homo sapiens RNA-seq of coding RNA Transcription profil..
The getAtlasData function allows to download the chosen RNA-Seq experiment
from the Expression Atlas and import it into a RangedSummarizedExperiment
object of a user's R session.
rse.hum <- getAtlasData('E-GEOD-67196')[[1]][[1]]
## Downloading Expression Atlas experiment summary from:
## ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/atlas/experiments/E-GEOD-67196/E-GEOD-67196-atlasExperimentSummary.Rdata
## Successfully downloaded experiment summary object for E-GEOD-67196
The design of the downloaded RNA-Seq experiment is described in the colData slot of
rse.hum. The following returns only its first five rows and columns.
colData(rse.hum)[1:5, 1:5]
## DataFrame with 5 rows and 5 columns
## AtlasAssayGroup organism individual organism_part
## <character> <character> <character> <character>
## SRR1927019 g1 Homo sapiens individual1 cerebellum
## SRR1927020 g2 Homo sapiens individual1 frontal cortex
## SRR1927021 g1 Homo sapiens individual2 cerebellum
## SRR1927022 g2 Homo sapiens individual2 frontal cortex
## SRR1927023 g1 Homo sapiens individual34 cerebellum
## disease
## <character>
## SRR1927019 amyotrophic lateral ..
## SRR1927020 amyotrophic lateral ..
## SRR1927021 amyotrophic lateral ..
## SRR1927022 amyotrophic lateral ..
## SRR1927023 amyotrophic lateral ..
The following example shows how to download from the above described SVG
repositories an aSVG image that matches the tissues and species
assayed in the gene expression data set downloaded in the previous subsection.
The return_feature function queries the repository for feature- and
species-related keywords, here c('frontal cortex', 'cerebellum') and c('homo sapiens', 'brain'), respectively. To return matching aSVGs, the argument
keywords.any is set to TRUE by default. When return.all=FALSE, only aSVGs
matching the query keywords are returned and saved under dir. Otherwise, all
aSVGs are returned regardless of the keywords. To avoid overwriting of existing
SVG files, it is recommended to start with an empty target directory, here
~/test. To search a local directory for matching aSVG images, the argument
setting remote=FALSE needs to be used, while specifying the path of the
corresponding directory under dir. All or only matching features are returned
if match.only is set to FALSE or TRUE, respectively.
dir.create('~/test') # Create empty directory
feature.df <- return_feature(feature=c('frontal cortex', 'cerebellum'), species=c('homo sapiens', 'brain'), keywords.any=TRUE, return.all=FALSE, dir='~/test', remote=TRUE, match.only=TRUE, desc=FALSE) # Query aSVGs
feature.df[1:8, ] # Return first 8 rows for checking
unique(feature.df$SVG) # Return all matching aSVGs
To build this vignettes according to the R/Bioconductor package requirements, the
following code section uses the aSVG file instance included in the
spatialHeatmap package rather than the downloaded instance from the previous
example.
feature.df <- return_feature(feature=c('frontal cortex', 'cerebellum'), species=c('homo sapiens', 'brain'), keywords.any=TRUE, return.all=FALSE, dir=svg.dir, remote=FALSE)
## Accessing features...
## arabidopsis_thaliana.organ_shm.svg, arabidopsis_thaliana.organ_shm1.svg, arabidopsis_thaliana.organ_shm2.svg, arabidopsis_thaliana.root.cross_shm.svg, arabidopsis_thaliana.root.ebi_shm.svg, arabidopsis_thaliana.root.roottip_shm.svg, arabidopsis_thaliana.shoot_shm.svg, arabidopsis_thaliana.shoot.root_shm.svg, gallus_gallus.svg, homo_sapiens.brain.svg, mus_musculus.male.svg, us_map_shm.svg,
Note, the target tissues frontal cortex and cerebellum are included in both
the experimental design slot of the downloaded expression data as well as the
annotations of the aSVG. This way these features can be colored in the downstream
SHM plots. If necessary users can also change from within R the feature identifiers
and names in an aSVG. Details on this utility are provided in the Supplementary Section.
feature.df
## feature id SVG parent index
## 1 middle frontal gyrus UBERON_0002702 homo_sapiens.brain.svg LAYER_EFO 8
## 2 cingulate cortex UBERON_0003027 homo_sapiens.brain.svg LAYER_EFO 21
## 3 prefrontal cortex UBERON_0000451 homo_sapiens.brain.svg LAYER_EFO 23
## 4 frontal cortex UBERON_0001870 homo_sapiens.brain.svg LAYER_EFO 24
## 5 cerebral cortex UBERON_0000956 homo_sapiens.brain.svg LAYER_EFO 25
## 6 cerebellum UBERON_0002037 homo_sapiens.brain.svg LAYER_EFO 27
## index1
## 1 6
## 2 19
## 3 21
## 4 22
## 5 23
## 6 25
Since the Expression Atlas supports the cross-species anatomy
ontology, the corresponding UBERON identifiers are
included in the id column of the data.frame returned by the above function
call of return_feature (Mungall et al. 2012). This ontology is also supported
by the rols Bioconductor package (Gatto 2019).
For organizing experimental designs and downstream plotting purposes, it can be
desirable to customize the text in certain columns of colData. This way one can
use the source data for displaying ‘pretty’ sample names in columns and legends
of all downstream tables and plots, respectively, in a consistent and automated
manner. To achieve this, the following example imports a ‘targets’ file that
can be generated and edited by the user in a text or spreadsheet program. In
the following example the target file content is used to replace the text in the
colData slot of the RangedSummarizedExperiment object with a version containing
shorter sample names that are more suitable for plotting purposes.
The following imports a custom target file containing simplified sample labels and experimental design information.
hum.tar <- system.file('extdata/shinyApp/example/target_human.txt', package='spatialHeatmap')
target.hum <- read.table(hum.tar, header=TRUE, row.names=1, sep='\t')
Load custom target data into colData slot.
colData(rse.hum) <- DataFrame(target.hum)
A slice of the simplified colData object is shown below, where the disease
column contains now shorter labels than in the original data set. Additional
details for generating and using target files in spatialHeatmap are provided
in the Supplementary Section of this vignette.
colData(rse.hum)[c(1:3, 41:42), 4:5]
## DataFrame with 5 rows and 2 columns
## organism_part disease
## <character> <character>
## SRR1927019 cerebellum ALS
## SRR1927020 frontal cortex ALS
## SRR1927021 cerebellum ALS
## SRR1927059 cerebellum normal
## SRR1927060 frontal cortex normal
The actual gene expression data of the downloaded RNA-Seq experiment is stored
in the assay slot of rse.hum. Since it contains raw count data, it can be
desirable to apply basic preprocessing routines prior to plotting spatial
heatmaps. The following shows how to normalize the count data, aggregate
replicates and then remove genes with unreliable expression responses. These
preprocessing steps are optional and can be skipped if needed. For this,
the expression data can be provided to the spatial_hm function directly, where
it is important to assign to the sam.factor and con.factor arguments
the corresponding sample and condition column names (Table 2).
For normalizing raw count data from RNA-Seq experiments, the norm_data
function can be used. It supports the following pre-existing functions from
widely used packages for analyzing count data in the next generation sequencing
(NGS) field: calcNormFactors (CNF) from edgeR (Robinson, McCarthy, and Smyth 2010); as well as
estimateSizeFactors (ESF), varianceStabilizingTransformation (VST), and
rlog from DESeq2 (Love, Huber, and Anders 2014). The argument norm.fun specifies one of the
four internal normalizing methods: CNF, ESF, VST, and rlog. If
norm.fun='none', no normalization is applied. The arguments for each
normalizing function are provided via a parameter.list, which is a list
with named slots. For example, norm.fun='ESF' and
parameter.list=list(type='ratio') is equivalent to
estimateSizeFactors(object, type='ratio'). If paramter.list=NULL, the
default arguments are used by the normalizing function assigned to norm.fun.
For additional details, users want to consult the help file of the norm_data
function by typing ?norm_data in the R console.
The following example uses the ESF normalization option. This method has been
chosen mainly due to its good time performance.
se.nor.hum <- norm_data(data=rse.hum, norm.fun='ESF', data.trans='log2')
## Normalising: ESF
## type
## "ratio"
Replicates are aggregated with the aggr_rep function, where the summary
statistics can be chosen under the aggr argument (e.g. aggr='mean'). The
columns specifying replicates can be assigned to the sam.factor and
con.factor arguments corresponding to samples and conditions, respectively.
For tracking, the corresponding sample/condition labels are used as column
titles in the aggregated assay instance, where they are concatenated with a
double underscore as separator. In addition, the corresponding rows in the
colData slot are collapsed accordingly.
se.aggr.hum <- aggr_rep(data=se.nor.hum, sam.factor='organism_part', con.factor='disease', aggr='mean')
## Syntactically valid column names are made!
assay(se.aggr.hum)[1:3, ]
## cerebellum__ALS frontal.cortex__ALS cerebellum__normal
## ENSG00000000003 7.024054 7.091484 6.406157
## ENSG00000000005 0.000000 1.540214 0.000000
## ENSG00000000419 7.866582 8.002549 8.073264
## frontal.cortex__normal
## ENSG00000000003 7.004446
## ENSG00000000005 1.403110
## ENSG00000000419 7.955709
To remove unreliable expression measures, filtering can be applied.
The following example retains genes with expression values
larger than 5 (log2 space) in at least 1% of all samples (pOA=c(0.01, 5)), and
a coefficient of variance (CV) between 0.30 and 100 (CV=c(0.30, 100)).
se.fil.hum <- filter_data(data=se.aggr.hum, sam.factor='organism_part', con.factor='disease', pOA=c(0.01, 5), CV=c(0.3, 100), dir=NULL)
## Syntactically valid column names are made!
To inspect the results, the following returns three selected rows of the fully preprocessed data matrix (Table 1).
assay(se.fil.hum)[c(5, 733:734), ]
| cerebellum__ALS | frontal.cortex__ALS | cerebellum__normal | frontal.cortex__normal | |
|---|---|---|---|---|
| ENSG00000006047 | 1.134172 | 5.2629629 | 0.5377534 | 5.3588310 |
| ENSG00000268433 | 5.324064 | 0.3419665 | 3.4780744 | 0.1340332 |
| ENSG00000268555 | 5.954572 | 2.6148548 | 4.9349736 | 2.0351776 |
The preprocessed expression values for any gene in the assay slot of
se.fil.hum can be plotted as an SHM. The following uses gene
ENSG00000268433 as an example. The chosen aSVG is a depiction of the human
brain where the assayed featured are colored by the corresponding expression
values in se.fil.hum.
shm.df <- spatial_hm(svg.path=svg.hum, data=se.fil.hum, ID=c('ENSG00000268433'), height=0.7, legend.r=1.5, legend.key.size=0.02, legend.text.size=12, legend.nrow=2)
## Coordinates: homo_sapiens.brain.svg ...
## Grobs: homo_sapiens.brain.svg ...
## SHMs and legend...
Figure 3: SHM of human brain
Only cerebellum and frontal cortex are colored, because they are present in both the aSVG and the expression data. The legend plot on the right maps the feature labels to the corresponding spatial regions in the image.
The plotting instructions of the SHM along with the corresponding
mapped features are stored as a list, here named shm.df. Its components
can be accessed as follows.
names(shm.df)
## [1] "spatial_heatmap" "mapped_feature"
# Mapped features
shm.df[['mapped_feature']]
## rowID featureSVG condition value SVG
## 1 ENSG00000268433 cerebellum ALS 5.3240638 homo_sapiens.brain.svg
## 2 ENSG00000268433 frontal.cortex ALS 0.3419665 homo_sapiens.brain.svg
## 3 ENSG00000268433 cerebellum normal 3.4780744 homo_sapiens.brain.svg
## 4 ENSG00000268433 frontal.cortex normal 0.1340332 homo_sapiens.brain.svg
In the above example, the normalized expression values of gene ENSG00000268433
are colored in the frontal cortex and cerebellum, where the different conditions,
here normal and ALS, are given in separate SHMs plotted next to
each other (Figure 3). The color and feature mappings are defined
by the corresponding color key and legend plot on the left and right, respectively.
SHMs for multiple genes can be plotted by providing the
corresponding gene IDs under the ID argument as a character vector. The
spatial_hm function will then sequentially arrange the SHMs for
each gene in a single composite plot. To facilitate comparisons among expression
values across genes and/or conditions, the lay.shm parameter can be assigned
'gene' or 'con', respectively. For instance, in Figure 4 the
SHMs of the genes ENSG00000268433 and ENSG00000006047 are organized
by condition in a horizontal view. This functionality is particularly useful when
comparing gene families. Users can also customize the order of the SHM subplots, by
assigning lay.shm='none'. With this setting the SHM subplots are organized according
to the gene and condition ordering under ID and data, respectively.
spatial_hm(svg.path=svg.hum, data=se.fil.hum, ID=c('ENSG00000268433', 'ENSG00000006047'), lay.shm='con', width=0.8, height=1, legend.r=1.5, legend.nrow=2)
## Coordinates: homo_sapiens.brain.svg ...
## Grobs: homo_sapiens.brain.svg ...
## SHMs and legend...
Figure 4: SHMs of two genes
The subplots are organized by “condition” with the lay.shm='con' setting.
SHMs can be saved to interactive HTML files as well as video files. To trigger
this export behavior the argument out.dir needs to be assinged a directory
path where the HTML, SVG image and video files will be stored. Each HTML file
contains an interactive SHM with zoom in and out functionality. Hovering over
graphics features will display data, gene, condition and other information. The
video will play the SHM subplots in the order specified under the lay.shm
argument.
The following example saves the interactive HTML and video files under the
a directory named ~/test.
if (!dir.exists('~/test')) dir.create('~/test')
spatial_hm(svg.path=svg.hum, data=se.fil.hum, ID=c('ENSG00000268433', 'ENSG00000006047'), lay.shm='con', width=0.8, height=1, legend.r=1.5, legend.nrow=2, out.dir='~/test')
To provide a high level of flexibility, the spatial_hm contains many arguments.
An overview of important arguments and their utility is provided in Table 2.
| argument | description |
|---|---|
| svg.path | Path of aSVG |
| data | Input data of SummarizedExperiment (SE), data frame, or vector |
| sam.factor | Applies to SE. Column name of sample replicates in colData slot. Default is NULL |
| con.factor | Applies to SE. Column name of condition replicates in colData slot. Default is NULL |
| ID | A character vector of row items for plotting spatial heatmaps |
| col.com | A character vector of color components for building colour scale. Default is c(‘yellow’, ‘orange’,‘red’) |
| col.bar | ‘selected’ or ‘all’, the former means use values of ID to build the colour scale while the latter use all values in data. Default is ‘selected’. |
| bar.width | A numeric of colour bar width. Default is 0.7 |
| data.trans | ‘log2’, ‘exp2’, or NULL, ‘log2’ transforms data to log2 scale for plotting while ‘exp2’ to 2-base exponent. Default is NULL, no transformation. |
| tis.trans | A vector of aSVG features to be transparent. Default is NULL. |
| width, height | Two numerics of overall width and height of all subplots, ranging between 0 and 1 repsectively. Default is 1, 1. |
| legend.r | A numeric to adjust the dimension of the legend plot. Default is 1. The larger, the higher ratio of width to height. |
| sub.title.size | The title size of each spatial heatmap subplot. Default is 11. |
| lay.shm | ‘gen’ or ‘con’, applies to multiple genes or conditions respectively. ‘gen’ means spatial heatmaps are organised by genes while ‘con’ organised by conditions. Default is ‘gen’ |
| ncol | The total column number of spatial heatmaps, not including legend plot. Default is 2. |
| sam.legend | ‘identical’, ‘all’, or a vector of samples/features in aSVG to show in legend plot. ‘identical’ only shows matching features while ‘all’ shows all features. |
| legend.ncol, legend.nrow | Two numbers of columns and rows of legend keys respectively. Default is NULL, NULL, since they are automatically set. |
| legend.position | the position of legend keys (‘none’, ‘left’, ‘right’,‘bottom’, ‘top’), or two-element numeric vector. Default is ‘bottom’. |
| legend.key.size, legend.text.size | The size of legend keys and labels respectively. Default is 0.5 and 8 respectively. |
| line.size, line.color | The size and colour of all plogyon outlines respectively. Default is 0.2 and ‘grey70’ respectively. |
| verbose | TRUE or FALSE. Default is TRUE and the aSVG features not mapped are printed to R console. |
| out.dir | The directory to save HTML and video files of spatial heatmaps. Default is NULL. |
This section generates an SHM plot for mouse data from the Expression Atlas. The code components are very similar to the previous Human Brain example. For brevity, the corresponding text explaining the code has been reduced to a minimum.
The chosen mouse RNA-Seq data compares tissue level gene expression across mammalian species (Merkin et al. 2012). The following searches the Expression Atlas for expression data from ‘heart’ and ‘Mus musculus’.
all.mus <- searchAtlasExperiments(properties="heart", species="Mus musculus")
## Searching for Expression Atlas experiments matching your query ...
## Query successful.
## Found 67 experiments matching your query.
Among the many matching entries, accession ‘E-MTAB-2801’ will be downloaded.
all.mus[7, ]
## DataFrame with 1 row and 4 columns
## Accession Species Type Title
## <character> <character> <character> <character>
## 1 E-MTAB-2801 Mus musculus RNA-seq of coding RNA Strand-specific RNA-..
rse.mus <- getAtlasData('E-MTAB-2801')[[1]][[1]]
## Downloading Expression Atlas experiment summary from:
## ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/atlas/experiments/E-MTAB-2801/E-MTAB-2801-atlasExperimentSummary.Rdata
## Successfully downloaded experiment summary object for E-MTAB-2801
The design of the downloaded RNA-Seq experiment is described in the colData slot of
rse.mus. The following returns only its first three rows.
colData(rse.mus)[1:3, ]
## DataFrame with 3 rows and 4 columns
## AtlasAssayGroup organism organism_part strain
## <character> <character> <character> <character>
## SRR594393 g7 Mus musculus brain DBA/2J
## SRR594394 g21 Mus musculus colon DBA/2J
## SRR594395 g13 Mus musculus heart DBA/2J
The following example shows how to download from the above described SVG
repositories an aSVG image that matches the tissues and species
assayed in the gene expression data set downloaded in the previous subsection.
As before the image is saved to a directory named ~/test.
if (!dir.exists('~/test')) dir.create('~/test')
feature.df <- return_feature(feature=c('heart', 'kidney'), species=c('Mus musculus'), keywords.any=TRUE, return.all=FALSE, dir='~/test', remote=TRUE, match.only=FALSE)
To build this vignettes according to the R/Bioconductor package requirements, the
following code section uses the aSVG file instance included in the
spatialHeatmap package rather than the downloaded instance from the example in
the previous step.
feature.df <- return_feature(feature=c('heart', 'kidney'), species=NULL, keywords.any=TRUE, return.all=FALSE, dir=svg.dir, remote=FALSE, match.only=FALSE)
## Accessing features...
## arabidopsis_thaliana.organ_shm.svg, arabidopsis_thaliana.organ_shm1.svg, arabidopsis_thaliana.organ_shm2.svg, arabidopsis_thaliana.root.cross_shm.svg, arabidopsis_thaliana.root.ebi_shm.svg, arabidopsis_thaliana.root.roottip_shm.svg, arabidopsis_thaliana.shoot_shm.svg, arabidopsis_thaliana.shoot.root_shm.svg, gallus_gallus.svg, homo_sapiens.brain.svg, mus_musculus.male.svg, us_map_shm.svg,
Return the names of the matching aSVG files.
unique(feature.df$SVG)
## [1] "gallus_gallus.svg" "mus_musculus.male.svg"
The following first selects mus_musculus.male.svg as target aSVG, then
returns the first three rows of the resulting feature.df, and finally prints
the unique set of all aSVG features.
feature.df <- subset(feature.df, SVG=='mus_musculus.male.svg')
feature.df[1:3, ]
## feature id SVG parent index index1
## 10 kidney UBERON_0002113 mus_musculus.male.svg LAYER_EFO 14 12
## 11 heart UBERON_0000948 mus_musculus.male.svg LAYER_EFO 51 49
## 12 path4204 path4204 mus_musculus.male.svg LAYER_OUTLINE 1 1
unique(feature.df[, 1])
## [1] "kidney" "heart"
## [3] "path4204" "aorta"
## [5] "circulatory system" "blood vessel"
## [7] "brown adipose tissue" "white adipose tissue"
## [9] "skin" "stomach"
## [11] "duodenum" "pancreas"
## [13] "spleen" "adrenal gland"
## [15] "colon" "small intestine"
## [17] "caecum" "jejunum"
## [19] "ileum" "esophagus"
## [21] "gall bladder" "parotid gland"
## [23] "submandibular gland" "lymph node"
## [25] "parathyroid gland" "tongue"
## [27] "Peyer’s patch" "prostate gland"
## [29] "vas deferens" "epididymis"
## [31] "testis" "seminal vesicle"
## [33] "penis" "urinary bladder"
## [35] "thymus" "femur"
## [37] "bone marrow" "cartilage"
## [39] "quadriceps femoris" "spinal cord"
## [41] "lung" "diaphragm"
## [43] "peripheral nervous system" "trachea"
## [45] "hindlimb" "trigeminal nerve"
## [47] "eye" "sciatic nerve"
## [49] "intestinal mucosa" "liver"
## [51] "brain" "skeletal muscle"
Obtain path of target aSVG on user system.
svg.mus <- system.file("extdata/shinyApp/example", "mus_musculus.male.svg", package="spatialHeatmap")
The following imports a sample target file that is included in this package. To inspect its content, the first three rows of the target file are printed to the screen.
mus.tar <- system.file('extdata/shinyApp/example/target_mouse.txt', package='spatialHeatmap')
target.mus <- read.table(mus.tar, header=TRUE, row.names=1, sep='\t')
target.mus[1:3, ]
## AtlasAssayGroup organism organism_part strain
## SRR594393 g7 Mus musculus brain DBA.2J
## SRR594394 g21 Mus musculus colon DBA.2J
## SRR594395 g13 Mus musculus heart DBA.2J
unique(target.mus[, 3])
## [1] "brain" "colon" "heart" "kidney"
## [5] "liver" "lung" "skeletal muscle" "spleen"
## [9] "testis"
Load custom target data into colData slot.
colData(rse.mus) <- DataFrame(target.mus)
The raw RNA-Seq count are preprocessed with the following steps: (1) normalization, (2) aggregation of replicates, and (3) filtering of reliable expression data. The details of these steps are explained in the sub-section above using data from human.
se.nor.mus <- norm_data(data=rse.mus, norm.fun='ESF', data.trans='log2') # Normalization
## Normalising: ESF
## type
## "ratio"
se.aggr.mus <- aggr_rep(data=se.nor.mus, sam.factor='organism_part', con.factor='strain', aggr='mean') # Aggregation of replicates
## Syntactically valid column names are made!
se.fil.mus <- filter_data(data=se.aggr.mus, sam.factor='organism_part', con.factor='strain', pOA=c(0.01, 5), CV=c(0.6, 100), dir=NULL) # Filtering of genes with low counts and variance
## Syntactically valid column names are made!
The pre-processed expression data for gene ‘ENSMUSG00000000263’ is plotted in form of an SHM. In this case the plot includes expression data for 8 tissues across 3 mouse strains.
spatial_hm(svg.path=svg.mus, data=se.fil.mus, ID=c('ENSMUSG00000000263'), height=0.7, legend.text.size=10, sub.title.size=9, ncol=3, tis.trans=c('skeletal muscle'), legend.nrow=4, line.size=0.2, line.color='grey70')
## Coordinates: mus_musculus.male.svg ...
## Grobs: mus_musculus.male.svg ...
## SHMs and legend...
Figure 5: SHM of mouse organs
This is a multiple-layer image where the shapes of the ‘skeletal muscle’ is set transparent to expose ‘lung’ and ‘heart’.
The SHM plots in Figures 5 and 6 demonstrate
the usage of the transparency feature via the tis.trans parameter. The
corresponding mouse organ aSVG image includes overlapping tissue layers. In
this case the skelectal muscle layer partially overlaps with lung and heart
tissues. To view lung and heart in Figure 5, the skelectal
muscle tissue is set transparent with tis.trans=c('skeletal muscle'). To view
in the same aSVG the skeletal muscle tissue instead, tis.trans is assigned
NULL for generating the SHM plot of Figure 6.
To fine control the visual effects in feature rich aSVGs, the line.size and
line.color parameters are useful. This way one can adjust the thickness and
color of complex structures.
spatial_hm(svg.path=svg.mus, data=se.fil.mus, ID=c('ENSMUSG00000000263'), height=0.6, legend.text.size=10, sub.title.size=9, ncol=3, tis.trans=NULL, legend.ncol=2, line.size=0.2, line.color='grey70')
## Coordinates: mus_musculus.male.svg ...
## Grobs: mus_musculus.male.svg ...
## SHMs and legend...
Figure 6: SHM of mouse organs
This is a multiple-layer image where the view onto ‘lung’ and ‘heart’ is obstructed by displaying the ‘skeletal muscle’ tissue.
This section generates an SHM plot for chicken data from the Expression Atlas. The code components are very similar to the Human Brain example. For brevity, the corresponding text explaining the code has been reduced to a minimum.
The chosen chicken RNA-Seq experiment compares the developmental changes across nine time points of seven organs (Cardoso-Moreira et al. 2019).
The following searches the Expression Atlas for expression data from ‘heart’ and ‘gallus’.
all.chk <- searchAtlasExperiments(properties="heart", species="gallus")
## Searching for Expression Atlas experiments matching your query ...
## Query successful.
## Found 3 experiments matching your query.
Among the matching entries, accession ‘E-MTAB-6769’ will be downloaded.
all.chk[3, ]
## DataFrame with 1 row and 4 columns
## Accession Species Type Title
## <character> <character> <character> <character>
## 1 E-MTAB-6769 Gallus gallus RNA-seq of coding RNA Chicken RNA-seq time..
rse.chk <- getAtlasData('E-MTAB-6769')[[1]][[1]]
## Downloading Expression Atlas experiment summary from:
## ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/atlas/experiments/E-MTAB-6769/E-MTAB-6769-atlasExperimentSummary.Rdata
## Successfully downloaded experiment summary object for E-MTAB-6769
The design of the downloaded RNA-Seq experiment is described in the colData
slot of rse.chk. The following returns only its first three rows.
colData(rse.chk)[1:3, ]
## DataFrame with 3 rows and 8 columns
## AtlasAssayGroup organism strain genotype
## <character> <character> <character> <character>
## ERR2576379 g1 Gallus gallus Red Junglefowl wild type genotype
## ERR2576380 g1 Gallus gallus Red Junglefowl wild type genotype
## ERR2576381 g2 Gallus gallus Red Junglefowl wild type genotype
## developmental_stage age sex organism_part
## <character> <character> <character> <character>
## ERR2576379 embryo 10 day female brain
## ERR2576380 embryo 10 day female brain
## ERR2576381 embryo 10 day female cerebellum
The following example shows how to download from the above introduced SVG
repositories an aSVG image that matches the tissues and species
assayed in the gene expression data set downloaded in the previous subsection.
As before the image is saved to a directory named ~/test.
# Make an empty directory "~/test" if not exist.
if (!dir.exists('~/test')) dir.create('~/test')
# Query aSVGs.
feature.df <- return_feature(feature=c('heart', 'kidney'), species=c('gallus'), keywords.any=TRUE, return.all=FALSE, dir='~/test', remote=TRUE, match.only=FALSE)
To build this vignettes according to the R/Bioconductor package requirements, the
following code section uses the aSVG file instance included in the
spatialHeatmap package rather than the downloaded instance from the previous
step.
feature.df <- return_feature(feature=c('heart', 'kidney'), species=c('gallus'), keywords.any=TRUE, return.all=FALSE, dir=svg.dir, remote=FALSE, match.only=FALSE)
## Accessing features...
## arabidopsis_thaliana.organ_shm.svg, arabidopsis_thaliana.organ_shm1.svg, arabidopsis_thaliana.organ_shm2.svg, arabidopsis_thaliana.root.cross_shm.svg, arabidopsis_thaliana.root.ebi_shm.svg, arabidopsis_thaliana.root.roottip_shm.svg, arabidopsis_thaliana.shoot_shm.svg, arabidopsis_thaliana.shoot.root_shm.svg, gallus_gallus.svg, homo_sapiens.brain.svg, mus_musculus.male.svg, us_map_shm.svg,
feature.df
## feature id SVG parent index
## 1 heart UBERON_0000948 gallus_gallus.svg LAYER_EFO 4
## 2 kidney UBERON_0002113 gallus_gallus.svg LAYER_EFO 5
## 3 chicken_outline chicken_outline gallus_gallus.svg LAYER_OUTLINE 1
## 4 brain UBERON_0000955 gallus_gallus.svg LAYER_EFO 3
## 5 liver UBERON_0002107 gallus_gallus.svg LAYER_EFO 6
## 6 skeletal muscle organ UBERON_0014892 gallus_gallus.svg LAYER_EFO 7
## 7 colon UBERON_0001155 gallus_gallus.svg LAYER_EFO 8
## 8 spleen UBERON_0002106 gallus_gallus.svg LAYER_EFO 9
## 9 lung UBERON_0002048 gallus_gallus.svg LAYER_EFO 10
## index1
## 1 2
## 2 3
## 3 1
## 4 1
## 5 4
## 6 5
## 7 6
## 8 7
## 9 8
Obtain path of target aSVG on user system.
svg.chk <- system.file("extdata/shinyApp/example", "gallus_gallus.svg", package="spatialHeatmap")
The following imports a sample target file that is included in this package. To inspect its content, the first three rows of the target file are printed to the screen.
chk.tar <- system.file('extdata/shinyApp/example/target_chicken.txt', package='spatialHeatmap')
target.chk <- read.table(chk.tar, header=TRUE, row.names=1, sep='\t')
target.chk[1:3, ]
## AtlasAssayGroup organism strain genotype
## ERR2576379 g1 Gallus gallus Red Junglefowl wild type genotype
## ERR2576380 g1 Gallus gallus Red Junglefowl wild type genotype
## ERR2576381 g2 Gallus gallus Red Junglefowl wild type genotype
## developmental_stage age sex organism_part
## ERR2576379 embryo day10 female brain
## ERR2576380 embryo day10 female brain
## ERR2576381 embryo day10 female cerebellum
Load custom target data into colData slot.
colData(rse.chk) <- DataFrame(target.chk)
Return samples used for plotting SHMs.
unique(colData(rse.chk)[, 'organism_part'])
## [1] "brain" "cerebellum" "heart" "kidney" "ovary"
## [6] "testis" "liver"
Return conditions considered for plotting downstream SHM.
unique(colData(rse.chk)[, 'age'])
## [1] "day10" "day12" "day14" "day17" "day0" "day155" "day35" "day7"
## [9] "day70"
The raw RNA-Seq count are preprocessed with the following steps: (1) normalization, (2) aggregation of replicates, and (3) filtering of reliable expression data. The details of these steps are explained in the above sub-section on human data.
se.nor.chk <- norm_data(data=rse.chk, norm.fun='ESF', data.trans='log2') # Normalization
## Normalising: ESF
## type
## "ratio"
se.aggr.chk <- aggr_rep(data=se.nor.chk, sam.factor='organism_part', con.factor='age', aggr='mean') # Replicate agggregation using mean
se.fil.chk <- filter_data(data=se.aggr.chk, sam.factor='organism_part', con.factor='age', pOA=c(0.01, 5), CV=c(0.6, 100), dir=NULL) # Filtering of genes with low counts and varince
The expression profile for gene ENSGALG00000006346 is plotted across nine time
points in four organs in form of a composite SHM with 9 panels. Their layout in
three columns is controlled with the argument setting ncol=3.
spatial_hm(svg.path=svg.chk, data=se.fil.chk, ID='ENSGALG00000006346', width=0.9, legend.width=0.9, legend.r=1.5, sub.title.size=9, ncol=3, legend.nrow=2, label=TRUE)
## Coordinates: gallus_gallus.svg ...
## Enrties not mapped: cerebellum, ovary, testis
## Grobs: gallus_gallus.svg ...
## SHMs and legend...
Figure 7: Time course of chicken organs
The SHM shows the expression profile of a single gene across nine time points and four organs.
This section generates an SHM for Arabidopsis thaliana tissues with gene expression
data from the Affymetrix microarray technology. The chosen experiment used
ribosome-associated mRNAs from several cell populations of shoots and roots that were
exposed to hypoxia stress (Mustroph et al. 2009). In this case the expression data
will be downloaded from GEO with utilites
from the GEOquery package (Davis and Meltzer 2007). The data preprocessing routines are
specific to the Affymetrix technology. The remaining code components for
generating SHMs are very similar to the previous examples. For brevity, the
text in this section explains mainly the steps that are specific to this data
set.
The GSE14502 data set will be downloaded with the getGEO function from the
GEOquery package. Intermediately, the expression data is stored in an
ExpressionSet container (Huber et al. 2015), and then converted to a
SummarizedExperiment object.
gset <- getGEO("GSE14502", GSEMatrix=TRUE, getGPL=TRUE)[[1]]
## Found 1 file(s)
## GSE14502_series_matrix.txt.gz
## Parsed with column specification:
## cols(
## .default = col_double(),
## ID_REF = col_character()
## )
## See spec(...) for full column specifications.
## File stored at:
## /tmp/RtmpgdRpz8/GPL198.soft
## Warning: 64 parsing failures.
## row col expected actual file
## 22747 SPOT_ID 1/0/T/F/TRUE/FALSE --Control literal data
## 22748 SPOT_ID 1/0/T/F/TRUE/FALSE --Control literal data
## 22749 SPOT_ID 1/0/T/F/TRUE/FALSE --Control literal data
## 22750 SPOT_ID 1/0/T/F/TRUE/FALSE --Control literal data
## 22751 SPOT_ID 1/0/T/F/TRUE/FALSE --Control literal data
## ..... ....... .................. ......... ............
## See problems(...) for more details.
se.sh <- as(gset, "SummarizedExperiment")
The gene symbol identifiers are extracted from the rowData component to be used
as row names. Similarly, one can work with AGI identifiers by providing below AGI
under Gene.Symbol.
rownames(se.sh) <- make.names(rowData(se.sh)[, 'Gene.Symbol'])
The following returns a slice of the experimental design stored in the
colData slot. Both the samples and conditions are contained in the title column.
The samples include promoters (pGL2, pCO2, pSCR, pWOL, p35S), tissues
and organs (root atrichoblast epidermis, root cortex meristematic zone, root
endodermis, root vasculature, root_total and shoot_total); and the conditions
are control and hypoxia.
colData(se.sh)[60:63, 1:4]
## DataFrame with 4 rows and 4 columns
## title geo_accession status
## <character> <character> <character>
## GSM362227 shoot_hypoxia_pGL2_r.. GSM362227 Public on Oct 12 2009
## GSM362228 shoot_hypoxia_pGL2_r.. GSM362228 Public on Oct 12 2009
## GSM362229 shoot_control_pRBCS_.. GSM362229 Public on Oct 12 2009
## GSM362230 shoot_control_pRBCS_.. GSM362230 Public on Oct 12 2009
## submission_date
## <character>
## GSM362227 Jan 21 2009
## GSM362228 Jan 21 2009
## GSM362229 Jan 21 2009
## GSM362230 Jan 21 2009
In this example, the aSVG image has been generated in Inkscape from
the corresponding figure in Mustroph et al. (2009). The resulting custom figure
has been included as a sample aSVG file in the spatialHeatmap package. Detailed
instructions for generating custom aSVG images in Inkscape are provided in the
SVG tutorial.
The annotations in the corresponding aSVG file located under svg.dir can be
queried with the return_features function.
feature.df <- return_feature(feature=c('pGL2', 'pRBCS'), species=c('shoot'), keywords.any=TRUE, return.all=FALSE, dir=svg.dir, remote=FALSE, match.only=FALSE)
## Accessing features...
## arabidopsis_thaliana.organ_shm.svg, arabidopsis_thaliana.organ_shm1.svg, arabidopsis_thaliana.organ_shm2.svg, arabidopsis_thaliana.root.cross_shm.svg, arabidopsis_thaliana.root.ebi_shm.svg, arabidopsis_thaliana.root.roottip_shm.svg, arabidopsis_thaliana.shoot_shm.svg, arabidopsis_thaliana.shoot.root_shm.svg, gallus_gallus.svg, homo_sapiens.brain.svg, mus_musculus.male.svg, us_map_shm.svg,
The unique set of the matching aSVG files can be returned as follows.
unique(feature.df$SVG)
## [1] "arabidopsis_thaliana.shoot_shm.svg"
## [2] "arabidopsis_thaliana.shoot.root_shm.svg"
The aSVG file arabidopsis_thaliana.shoot_shm.svg is chosen to generate the SHM in this section.
feature.df <- subset(feature.df, SVG=='arabidopsis_thaliana.shoot_shm.svg')
feature.df[1:3, ]
## feature id SVG parent index
## 1 shoot_pGL2 shoot_pGL2 arabidopsis_thaliana.shoot_shm.svg container 2
## 2 shoot_pRBCS shoot_pRBCS arabidopsis_thaliana.shoot_shm.svg container 3
## 3 g258 g258 arabidopsis_thaliana.shoot_shm.svg container 1
## index1
## 1 2
## 2 3
## 3 1
Obtain full path of target aSVG on user system.
svg.sh <- system.file("extdata/shinyApp/example", "arabidopsis_thaliana.shoot_shm.svg", package="spatialHeatmap")
The following imports a sample target file that is included in this package. To inspect its content, four selected rows of this target file are printed to the screen.
sh.tar <- system.file('extdata/shinyApp/example/target_arab.txt', package='spatialHeatmap')
target.sh <- read.table(sh.tar, header=TRUE, row.names=1, sep='\t')
target.sh[60:63, ]
## col.name samples conditions
## shoot_hypoxia_pGL2_rep1 GSM362227 shoot_pGL2 hypoxia
## shoot_hypoxia_pGL2_rep2 GSM362228 shoot_pGL2 hypoxia
## shoot_control_pRBCS_rep1 GSM362229 shoot_pRBCS control
## shoot_control_pRBCS_rep2 GSM362230 shoot_pRBCS control
Return all samples present in target file.
unique(target.sh[, 'samples'])
## [1] "root_total" "root_p35S" "root_pSCR" "root_pSHR"
## [5] "root_pWOL" "root_pGL2" "root_pSUC2" "root_pSultr2.2"
## [9] "root_pCO2" "root_pPEP" "root_pRPL11C" "shoot_total"
## [13] "shoot_p35S" "shoot_pGL2" "shoot_pRBCS" "shoot_pSUC2"
## [17] "shoot_pSultr2.2" "shoot_pCER5" "shoot_pKAT1"
Return all conditions present in target file.
unique(target.sh[, 'conditions'])
## [1] "control" "hypoxia"
Load custom target data into colData slot.
colData(se.sh) <- DataFrame(target.sh)
The downloaded GSE14502 data set has already been normalized with the RMA algorithm (Gautier et al. 2004). Thus, the pre-processing steps can be restricted to the aggregation of replicates and filtering of reliably expressed genes. For the latter, the following code will retain genes with expression values larger than 6 (log2 space) in at least 3% of all samples (pOA=c(0.03, 6)), and with a coefficient of variance (CV) between 0.30 and 100 (CV=c(0.30, 100)).
se.aggr.sh <- aggr_rep(data=se.sh, sam.factor='samples', con.factor='conditions', aggr='mean') # Replicate agggregation using mean
se.fil.arab <- filter_data(data=se.aggr.sh, sam.factor='samples', con.factor='conditions', pOA=c(0.03, 6), CV=c(0.30, 100), dir=NULL) # Filtering of genes with low intensities and variance
The expression profile for the HRE2 gene is plotted for control and hypoxia treatment across six cell types (Figure 8).
spatial_hm(svg.path=svg.sh, data=se.fil.arab, ID=c("HRE2"), height=0.7, legend.nrow=3, legend.text.size=11)
## Coordinates: arabidopsis_thaliana.shoot_shm.svg ...
## Enrties not mapped: root_total, root_p35S, root_pSCR, root_pSHR, root_pWOL, root_pGL2, root_pSUC2, root_pSultr2.2, root_pCO2, root_pPEP, root_pRPL11C, shoot_total, shoot_p35S
## Grobs: arabidopsis_thaliana.shoot_shm.svg ...
## SHMs and legend...
Figure 8: SHM of Arabidopsis shoots
The expression profile of the HRE2 gene is plotted for control and hypoxia treatment across six cell types.
The spatial_hm function can arrange multiple aSVGs in a single SHM plot, such
as aSVGs from different development stages. To organize the subplots, the names
of the aSVG files are expected to include the following suffixes: *_shm1.svg,
*_shm2.svg, etc. The paths to the aSVG files are provided under the
svg.path argument. By default, every aSVG image will have a legend plot on
the right. The legend argument provides fine control over which legend plots
to display.
As a simple toy example, the following stores random numbers in a
data.frame.
df.random <- data.frame(matrix(sample(x=1:100, size=50, replace=TRUE), nrow=10))
colnames(df.random) <- c('shoot_totalA__condition1', 'shoot_totalA__condition2', 'shoot_totalB__condition1', 'shoot_totalB__condition2', 'notMapped') # Assign column names
rownames(df.random) <- paste0('gene', 1:10) # Assign row names
df.random[1:3, ]
## shoot_totalA__condition1 shoot_totalA__condition2
## gene1 19 73
## gene2 28 5
## gene3 52 52
## shoot_totalB__condition1 shoot_totalB__condition2 notMapped
## gene1 62 100 63
## gene2 13 80 87
## gene3 20 24 34
Obtain paths to aSVG files, here for younger and older plants using *_shm1 and
*_shm1, respectively.
svg.sh1 <- system.file("extdata/shinyApp/example", "arabidopsis_thaliana.organ_shm1.svg", package="spatialHeatmap")
svg.sh2 <- system.file("extdata/shinyApp/example", "arabidopsis_thaliana.organ_shm2.svg", package="spatialHeatmap")
The following generates the corresponding SHMs plot for gene1. The orginal
image dimensions can be preserved by assigning TRUE to the preserve.scale argument.
spatial_hm(svg.path=c(svg.sh1, svg.sh2), data=df.random, ID=c('gene1'), width=0.7, legend.r=0.9, legend.width=1, preserve.scale=TRUE)
## Coordinates: arabidopsis_thaliana.organ_shm1.svg ...
## Coordinates: arabidopsis_thaliana.organ_shm2.svg ...
## Enrties not mapped: shoot_totalB, notMapped
## Enrties not mapped: shoot_totalA, notMapped
## Grobs: arabidopsis_thaliana.organ_shm1.svg ...
## Grobs: arabidopsis_thaliana.organ_shm2.svg ...
## SHMs and legend...
Figure 9: SHMs of Arabidopsis at two growth stages
The expression profile of gene1 under condition1 and condition2 is plotted for two growth stages (top and bottom row).
SHMs are a visualization approach suitable for comparing the expression profiles
of single genes or a small number of them across cell types and conditions. To
also support analyses across larger number of genes, spatialHeatmap provides
utilities for identifying for gene(s) of interest nearest neighbor genes that share
similar expression profiles. This is achieved by identifying clusters and
network modules using hierarchical clustering and network analysis, and the former is displayed as matrix heatmap while the latter as network graph . These approaches
are described with the Arabidopsis Shoot data in this and the following sections, respectively.
The nearest neighbors of target genes are selected based on the correlation coefficient or distance between genes, where the default is Pearson correlation coefficient (PCC). The argument p is the proportion of genes showing most similar expression profiles with target genes. Only genes within this proportion are returned. Other two alternative threshold arguments are n and v. The former selects top n most similar genes, while the latter is a real PCC or distance value and only genes within this value are selected. Note, the nearest neighbors are selected for each target gene independently. In this example, the target genes are RCA and HRE2. The argument ann is the column name of gene annotation in rowData slot. It is only relevant if users want to see annotation when mousing over a node in the interactive network below, so it is optional to be assigned a value. Here ann='Target.Description' is set and the corresponding annotation is appended to selected nearest neighbors.
sub.mat <- submatrix(data=se.fil.arab, ann='Target.Description', ID=c('RCA', 'HRE2'), p=0.1)
The subsetted matrix (target genes and nearest neighbors) and the complete PCC matrix are returned in a list, and partial is shown below.
sub.mat[['sub_matrix']][c('RCA', 'HRE2'), c(1:3, 37)] # Subsetted data matrix.
## root_total__control root_total__hypoxia root_p35S__control
## RCA 6.569305 6.416811 7.443822
## HRE2 5.486920 11.370161 5.578123
## Target.Description
## RCA hypothetical protein ;supported by full-length cDNA: Ceres:7114.
## HRE2 putative AP2 domain transcription factor
sub.mat[['cor_dist']][1:3, 1:3] # Correlation matrix.
## ndhA petL psaJ
## ndhA 1.0000000 0.8991317 0.9189853
## petL 0.8991317 1.0000000 0.9585802
## psaJ 0.9189853 0.9585802 1.0000000
Hierarchical clustering is applied on the subsetted matrix and results are displayed as matrix heatmap in either static or interactive mode. Figure 10 is the static mode on gene RCA and HRE2. The rows (genes) and columns (samples) are sorted by hierarchical clustering dendrograms. Target genes are labeled by black lines. It is manifest that the two target genes belong to two separate patterns respectively. Setting static=FALSE launches the interactive mode, where users can zoom in for details by drawing a rectangle, and zoom out by double clicking the heatmap.
matrix_hm(ID=c('RCA', 'HRE2'), data=sub.mat[['sub_matrix']], angleCol=80, angleRow=35, cexRow=0.8, cexCol=0.8, margin=c(10, 6), static=TRUE, arg.lis1=list(offsetRow=0.01, offsetCol=0.01))
Figure 10: Matrix Heatmap
Rows are genes and columns are samples. The input genes are tagged by black lines.
In this section, network analysis is applied on the subsetted gene expression matrix from last section. It includes network module identification and visualization of modules in form of network graphs.
jianhai-reply: the blue text are responses to ThG-Comments.
[ThG-Comment: this section needs major improvements. The intent was to identify for a target gene nearest neighbors based on some similarity metric for gene expression profiles (e.g. correlation), and then hierarchically cluster them and visualize the result in a heatmap. Instead you are subjecting the entire expression matrix to clustering/WGCNA and then plot the target gene in the context of a single cluster/module. This is hugely inefficient compared to doing it only for the nearest neighbors instead. Just think about what will happen if a user imports an expression matrix for all transcripts from human. With your approach they will not be able to complete this step since they will run out of memory on their system.]
The WGCNA network analysis (Langfelder and Horvath 2008) is applied on the subsetted gene expression matrix to identify gene modules, which are clusters of genes showing highly similar expression profiles. First, a correlation matrix or distance matrix is computed on the subsetted matrix, which defaults to Pearson correlation or euclidean distance respectively. Then the computed matrix is transformed to an adjacency matrix. The adjacency matrix is characterized by sale-free topology, where only a small number of genes are expected to have many connections with other genes and thus dominate the whole network (Ravasz et al. 2002). Values in this adjacency matrix denotes expression similarity between genes. The larger value, the higher similarity.1 You need to describe how this adjency matrix is generated and what it represents. It also needs to mention what type of disance/correlation method is used initially to generate the downstream matrix.2 Without defining how adjacency is defined here readers will not be able to understand what was done here, especially since this section is mainly about hierarchical clustering.
Next, the adjacency matrix is used to calculate an advanced similarity measure of topological overlap matrix (TOM), which quantifies expression similarity for a pair of genes in the context of all other genes. Then, the TOM transformed distance matrix 1-TOM is used for hierarchical clustering with flashClust (Langfelder and Horvath 2012). Lastly, network modules are identified with the dynamicTreeCut package (Langfelder, Zhang, and Steve Horvath 2016). It provides an argument deepSplit (ds) for rough control over sensitivity to module splitting. The ds has four alternative values 0, 1, 2, 3, and each generates an alternative set of modules. The higher the value, the more and smaller modules are produced, thus there are four alternative sets of modules generated with varying sizes.3 What is the meaning of the sensitivity levels, how are they
generated and how should the user interpret them?4 Unclear for what you need
WGCNA here. I thought this section uses hierarchical clustering. The latter is
certainly used by WGCNA but it remains a mystery what exactly you are doing
here. I would expect to use here only hierarchical clustering, e.g. with
flashClust. WGCNA seems more suitable for the next section.
The whole process above of identifying modules is implemented in the function adj_mod. It returns a list containing the adjacency matrix and a data frame of module assignment. Since the interactive network below performs better on smaller modules, only modules resulting from ds=2 and ds=3 are returned.
adj.mod <- adj_mod(data=sub.mat[['sub_matrix']])
Partial of the adjacency matrix is shown below.
adj.mod[['adj']][1:3, 1:3]
## CA1 PSAH.1 AT2G26500
## CA1 1.0000000 0.9514016 0.9636366
## PSAH.1 0.9514016 1.0000000 0.9611725
## AT2G26500 0.9636366 0.9611725 1.0000000
The module assignment is a data frame. The first column is ds=2 while the second is ds=3. The numbers in each column are module labels with 0 meaning genes not assigned to any modules. First three rows are shown below.
adj.mod[['mod']][1:3, ]
## 2 3
## CA1 1 1
## PSAH.1 1 1
## AT2G26500 1 1
[ThG-Comment: both the previous section and this section need to clearly explain what is done to arrive at a given network module and what it represents. Once this is done I will edit the text.]
To visualize modules, a target gene should be selected first, usually a gene from the SHMs. Then the module containing the target gene is internally selected based on the ds, which defaults to 3. There are two modes to visualize the selected module, static or interactive. Figure 11 is the static network containing gene HRE2, which is generated by static=TRUE. Nodes are genes and edges are adjacencies between genes. The thicker edge denotes higher adjacency while larger node indicates higher gene connectivity (sum of a gene's adjacency with all its direct neighbours). The target gene is labeled by ’_target’.
network(ID="HRE2", data=sub.mat[['sub_matrix']], adj.mod=adj.mod, adj.min=0.90, vertex.label.cex=1.2, vertex.cex=2, static=TRUE)
Figure 11: Static network
Node size denotes gene connectivity while edge thickness stands for co-expression similarity.
Setting static=FALSE launches the interactive network. There is an interactive color bar to denote gene connectivity. The color ingredients must only be separated by comma, e.g. yellow,orange,red, which means gene connectivity increases from yellow to red. If too many edges (e.g.: > 300) are displayed, the network could get stuck. So the ‘Adjacency threshold’ option sets a threthold to filter out weak edges. If not too many edges retained (e.g.: < 300), users can check ‘Yes’ under ‘Show plot’, then the network would be responsive smoothly. To maintain acceptable performance, users are advised to choose a stringent threshold (e.g. 0.9) initially, then decrease the value gradually. The interactive feature allows users to zoom in and out, or drag a gene around. All the gene IDs in the network module are listed in ‘Select by id’ in decreasing order according to gene connectivity. Same with static mode, the target gene ID is appended ’_target’.
If gene annotation is appended as a column in the subsetted expression matrix, it will be detected by the function network automatically, and is seen by mousing over a node.
network(ID="HRE2", data=sub.mat[['sub_matrix']], adj.mod=adj.mod, static=FALSE)
In additon to generating SHMs and the corresponding gene context plots from R,
spatialHeatmap includes a Shiny App that
provides access to all the functionalities from an intuitive-to-use web
browser interface. Apart from being very user-friendly, this App
conveniently organizes the results of the entire visualization workflow in a
single browser window with options to adjust the parameters of the individual components
interactively. For instance, genes can be selected and replotted in the SHM
simply by clicking the corresponding rows in the expression table included in the same window. The subplots of SHMs, including multiple growth stages, are presented in three modes “Basic”, “Animation”, and “Video”. The “Basic” compiles all subplots in the same page, and the dimension, layout, and color key are all customizable, the “Animation” displays each subplot sequentially in an animation. The animation can be played continuously or paused at a frame. Each frame can be zoomed in and out by drawing a rectangle and clicking the “home” icon in the top-right toolbar respectively. When mouse over a shape, the information of gene, condition, feature identifier, and value are displayed in a tootip, while the “Video” assembles all SHMs in am MP4 file. This representation is very efficient in guiding the interpretation of the results
in a visual and user-friendly manner. For testing purposes, the spatialHeatmap
Shiny App also includes ready-to-use sample expression data and aSVG images
along with embedded user instructions.
The Shiny App of spatialHeatmap can be launched from an R session with the following
function call.
shiny_all()
[ThG-Comment: many menu items are not functional in the Shiny App. E.g.: Instructions and Acknowledgement return error messages.] jianhai-reply: Missing pre-uploaded files are added, and now it is functional.
The dashboard panels of the Shiny App are organized as follows:
The parameters for controlling each functionality are placed on top of respective panel.
A screenshot of the Spatial Heatmap component within the Shiny App window is shown below (Figure 12).
Figure 12: Screenshot of spatialHeatmap's Shiny App
This the Spatial Heatmap component. The generated SHMs are one of the pre-uploaded examples.
After launching, the Shiny App displays by default one of the included data sets.
The gene expression data and aSVG image are uploaded to the Shiny App
as tabular text (e.g. in CSV or TSV format) and SVG file, respectively. To
also allow users to upload gene expression data stored in
SummarizedExperiment objects, one can export them from R to a tabular file
with the filter_data function. In this function call, the user sets the
desired directory path under dir. Within this directory the tabular file will
be written to local_mode_result/processed_data.txt in TSV format. The
column names in the exported tabular file preserve the experimental design information from
the colData slot by concatenating the corresponding sample and condition information
separated by double underscores. An example of this format is shown in Table 1.
To interactively access gene- or transcript-level annotations in the plots and
tables of the Shiny App, such as viewing functional descriptions by moving the
cursor over network nodes, the corresponding annotation column needs to be
present in the rowData slot and its column name assigned to the ann
argument. In the exported tabular file the extra annotation column is appended
to the expression matrix.
jianhai-reply: yes, that’s fine. 5 Check for correctness. For readability I made a lot of changes in this paragraph.
se.fil.arab <- filter_data(data=se.aggr.sh, ann="Target.Description", sam.factor='samples', con.factor='conditions', pOA=c(0.03, 6), CV=c(0.30, 100), dir='./')
As most Shiny Apps, spatialHeatmap can be deployed as a centralized web
service. A major advantage of a web server deployment is that the
functionalities can be accessed remotely by anyone on the internet without the
need to use R on the user system. For deployment one can use custom web
servers or cloud services, such as AWS, GCP or
shinysapps.io. An example web instance for testing
spatialHeatmap online is available
here.
Lastly, in either local or online deployment there is a potential computation issue if the tabular file contains too many rows such as more than 10,000. Specifically, too many rows would cause intensive computation in the network analysis and potentially results in system collapse. To prevent this potential issue, the Shiny App is added an option of local work mode. So users can get the intensive network analysis computed outside Shiny and then upload the computed results to the local mode. Note, in this case three files should be uploaded: filtered data matrix, adjacency matrix, module assignment, where the last two files should be generated on the first one. The details are documented in help files of function filter_data and adj_mod.
To generate SHMs with custom data, proper formatting of the numeric (assay) data and aSVG images is required. A tabular target file describing the numeric data can also be provided but is optional. This section provides additional details on these three input types.
The numceric data used to color the features in aSVG images can be provided as
three different object types including vector, data.frame and
SummerizedExperiment. When working with complex omics-based assay data then
the latter provides the most flexibility, and thus should be the preferred
container class for managing numeric data in spatialHeatmap. Both
data.frame and SummarizedExperiment can hold data from many measured items,
such as genes across many samples and conditions. In contrast to this, the
vector class is only suitable for data from single items. Due to its
simplicity this less complex container is often useful for testing or when
dealing with simple data sets.
vectorWhen using numeric vectors as input to spatial_hm, then their name slot needs
to be populated with strings matching the feature names in the corresponding aSVG.
To also specify conditions, their labels need to be appended to the feature names
with double underscores as separator, i.e. ’feature__condition’.
The following example replots the toy example for two spatial features (‘occipital lobe’ and ‘parietal lobe’) and two conditions (‘1’ and ‘2’).
vec <- sample(x=1:100, size=5) # Random numeric values
names(vec) <- c('occipital lobe__condition1', 'occipital lobe__condition2', 'parietal lobe__condition1', 'parietal lobe__condition2', 'notMapped') # Assign unique names to random values
vec
## occipital lobe__condition1 occipital lobe__condition2
## 16 78
## parietal lobe__condition1 parietal lobe__condition2
## 43 19
## notMapped
## 7
With this configuration the resulting plot contains two spatial heatmap plots
for the human brain, one for ‘condition 1’ and another one for ‘contition 2’.
To keep the build time and storage size of this package to a minimum, the
spatial_hm function call in the code block below is not evaluated. Thus,
the corresponding SHM is not shown in this vignette.
spatial_hm(svg.path=svg.hum, data=vec, ID='toy', ncol=1, legend.r=1.2, sub.title.size=14)
data.frameCompared to the above vector input, data.frames are structured here like row-wise
appended vectors, where the name slot information in the vectors is stored in the
column names. Each row also contains a name that corresponds to the corresponding
item name, such as a gene ID. The naming of spatial features and conditions in the
column names follows the same conventions as the naming used for the name slots in
the above vector example.
The following illustrates this with an example where a numeric data.frame with
random numbers is generated containing 20 rows and 5 columns. To avoid name clashes,
the values in the rows and columns should be unique.
df.test <- data.frame(matrix(sample(x=1:1000, size=100), nrow=20)) # Create numeric data.frame
colnames(df.test) <- names(vec) # Assign column names
rownames(df.test) <- paste0('gene', 1:20) # Assign row names
df.test[1:3, ]
## occipital lobe__condition1 occipital lobe__condition2
## gene1 510 398
## gene2 452 718
## gene3 396 790
## parietal lobe__condition1 parietal lobe__condition2 notMapped
## gene1 602 610 684
## gene2 764 18 283
## gene3 78 109 801
With the resulting data.frame one can generate the same SHM as in the previous
example, that used a named vector as input to the spatial_hm function. Additionally,
one can now select each row by its name (here gene ID) under the ID argument.
spatial_hm(svg.path=svg.hum, data=df.test, ID=c('gene1'), ncol=1, legend.r=1.2, sub.title.size=14)
Additional information can be appended to the data.frame column-wise, such as
annotation data including gene descriptions. This information can then be displayed
interactively in the network plots of the Shiny App by placing the curser over
network nodes.
df.test$ann <- paste0('ann', 1:20)
df.test[1:3, ]
## occipital lobe__condition1 occipital lobe__condition2
## gene1 510 398
## gene2 452 718
## gene3 396 790
## parietal lobe__condition1 parietal lobe__condition2 notMapped ann
## gene1 602 610 684 ann1
## gene2 764 18 283 ann2
## gene3 78 109 801 ann3
SummarizedExperimentThe SummarizedExperiment class is a much more extensible and flexible container
for providing metadata for both rows and columns of numeric data stored in tabular
format.
To import experimental design information from tabular files, users can provide
a target file that will be stored in the colData slot of the
SummarizedExperiment (SE, Morgan et al. (2018)) object. In other words, the
target file provides the metadata for the columns of the numeric assay data. Usually,
the target file contains at least two columns: one for the features/samples and
one for the conditions. Replicates are indicated by identical entries in these
columns. The actual numeric matrix representing the assay data is stored in
the assay slot, where the rows correspond to items, such as gene IDs.
Optionally, additional annotation information for the rows (e.g. gene
descriptions) can be stored in the rowData slot.
For constructing a valid SummarizedExperiment object, that can be used by
the spatial_hm function, the target file should meet the following requirements.
It should be imported with read.table or read.delim into a data.frame
or the data.frame can be constructed in R on the fly (as shown below).
It should contain at least two columns. One column represents the features/samples
and the other one the conditions. The rows in the target file
correspond to the columns of the numeric data stored in the assay slot. If
the condition column is empty, then the same condition is assumed under the
corresponding features/samples entry.
The feature/sample names must have matching entries in to corresponding aSVG to be considered in the final SHM. Note, the double underscore is a special string that is reserved for specific purposes in spatialHeatmap, and thus should be avoided for naming feature/samples and conditions.
The following example illustrates the design of a valid SummarizedExperiment
object for generating SHMs. In this example, the ‘occipital lobe’ tissue has 2
conditions and each condition has 2 replicates. Thus, there are 4 assays for
occipital lobe, and the same design applies to the parietal lobe tissue.
sample <- c(rep('occipital lobe', 4), rep('parietal lobe', 4))
condition <- rep(c('condition1', 'condition1', 'condition2', 'condition2'), 2)
target.test <- data.frame(sample=sample, condition=condition, row.names=paste0('assay', 1:8))
target.test
## sample condition
## assay1 occipital lobe condition1
## assay2 occipital lobe condition1
## assay3 occipital lobe condition2
## assay4 occipital lobe condition2
## assay5 parietal lobe condition1
## assay6 parietal lobe condition1
## assay7 parietal lobe condition2
## assay8 parietal lobe condition2
The assay slot is populated with a 8 x 20 data.frame containing random
numbers. Each column corresponds to an assay in the target file (here imported
into colData), while each row corresponds to a gene.
df.se <- data.frame(matrix(sample(x=1:1000, size=160), nrow=20))
rownames(df.se) <- paste0('gene', 1:20)
colnames(df.se) <- row.names(target.test)
df.se[1:3, ]
## assay1 assay2 assay3 assay4 assay5 assay6 assay7 assay8
## gene1 342 417 324 523 626 364 180 463
## gene2 789 68 258 45 237 565 29 225
## gene3 838 114 231 654 139 693 819 990
Next, the final SummarizedExperiment object is constructed by providing the
numeric and target data under the assays and colData arguments,
respectively.
se <- SummarizedExperiment(assays=df.se, colData=target.test)
se
## class: SummarizedExperiment
## dim: 20 8
## metadata(0):
## assays(1): ''
## rownames(20): gene1 gene2 ... gene19 gene20
## rowData names(0):
## colnames(8): assay1 assay2 ... assay7 assay8
## colData names(2): sample condition
If needed row-wise annotation information (e.g. for genes) can be included in
the SummarizedExperiment object as well. This can be done during the
construction of the initial object, or as below by injecting the information
into an existing SummarizedExperiment object.
rowData(se) <- df.test['ann']
In this simple example, possible normalization and filtering steps are skipped. Yet, the aggregation of replicates is performed as shown below.
se.aggr <- aggr_rep(data=se, sam.factor='sample', con.factor='condition', aggr='mean')
## Syntactically valid column names are made!
assay(se.aggr)[1:3, ]
## occipital.lobe__condition1 occipital.lobe__condition2
## gene1 379.5 423.5
## gene2 428.5 151.5
## gene3 476.0 442.5
## parietal.lobe__condition1 parietal.lobe__condition2
## gene1 495 321.5
## gene2 401 127.0
## gene3 416 904.5
With the fully configured SummarizedExperiment object, a similar SHM is plotted as
in the previous examples.
spatial_hm(svg.path=svg.hum, data=se.aggr, ID=c('gene1'), ncol=1, legend.r=1.2, sub.title.size=14)
An aSVG repository, that can be used by spatialHeatmap directly, has been
generated by the EBI Gene Expression
Group. It
contains annatomical aSVG images from different species. These SVG images are
also used by the Expression Atlas database. In addition, the spatialHeatmap has its own
repository called spatialHeatmap aSVG Repository, where some aSVG files developed in this project are already deposited (e.g. Figure 8).6 It
is confusing that you are not mentioning here your custom aSVG repos.
If users cannot find a target aSVG in the two repositories, we have developed a step-by-step SVG tutorial for creating custom aSVG images. For example, the BAR eFP browser at University of Toronto contains many anatomical images, and these images can be used as templates in the SVG tutorial to make custom aSVGs.
We will add more aSVGs to our repository in the future and users are welcome to deposit their own aSVGs there to share with other spatialHeatmap users.
To create and edit existing feature identifiers in aSVGs, the update_feature function
can be used. The demonstration below, creates an empty folder ~/test1 and copies
into it the homo_sapiens.brain.svg aSVG image provided by the spatialHeatmap
package. Subsequently, selected feature annotations are updated in this file.
if (!dir.exists('~/test1')) dir.create('~/test1') # Create an empty directory
svg.hum <- system.file("extdata/shinyApp/example", 'homo_sapiens.brain.svg', package="spatialHeatmap")
file.copy(from=svg.hum, to='~/test1', overwrite=FALSE) # Copy "homo_sapiens.brain.svg" file into '~/test1'
Query the above aSVG with feature and species keywords, and return the resulting matches
in a data.frame.
feature.df <- return_feature(feature=c('frontal cortex'), species=c('homo sapiens', 'brain'), dir='~/test1', remote=FALSE, keywords.any=FALSE)
feature.df
Subsequently, create a character vector of new feature identifiers corresponding to
each of the returned features. In the following examples spaces in strings will be
filled with dots. This character vector must be added to the first column of the
feature data.frame. The latter is used by the update_feature function to look
up new features.
Sample code that creates new feature names and stores them in a character vector.
f.new <- c('frontal.cortex', 'prefrontal.cortex')
Next, new features are added to the first column of the feature data.frame.
feature.df.new <- cbind(featureNew=f.new, feature.df)
feature.df.new
Finally, the features are updated in the aSVG file(s) located in the ~/test1 directory.
update_feature(feature=feature.df.new, dir='~/test1')
sessionInfo()
## R Under development (unstable) (2020-05-30 r78619)
## Platform: x86_64-pc-linux-gnu (64-bit)
## Running under: Debian GNU/Linux 10 (buster)
##
## Matrix products: default
## BLAS: /usr/lib/x86_64-linux-gnu/atlas/libblas.so.3.10.3
## LAPACK: /usr/local/lib/R-devel/lib/R/lib/libRlapack.so
##
## locale:
## [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
## [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
## [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
## [7] LC_PAPER=en_US.UTF-8 LC_NAME=C
## [9] LC_ADDRESS=C LC_TELEPHONE=C
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
##
## attached base packages:
## [1] stats4 parallel stats graphics grDevices utils datasets
## [8] methods base
##
## other attached packages:
## [1] GEOquery_2.57.0 ExpressionAtlas_1.17.0
## [3] xml2_1.3.2 limma_3.45.0
## [5] SummarizedExperiment_1.19.6 DelayedArray_0.15.6
## [7] matrixStats_0.56.0 Matrix_1.2-18
## [9] Biobase_2.49.0 GenomicRanges_1.41.5
## [11] GenomeInfoDb_1.25.8 IRanges_2.23.10
## [13] S4Vectors_0.27.12 BiocGenerics_0.35.2
## [15] spatialHeatmap_0.99.0 knitr_1.28
## [17] BiocStyle_2.17.0
##
## loaded via a namespace (and not attached):
## [1] backports_1.1.7 rols_2.17.1 Hmisc_4.4-0
## [4] av_0.5.0 igraph_1.2.5 lazyeval_0.2.2
## [7] shinydashboard_0.7.1 splines_4.1.0 BiocParallel_1.23.0
## [10] ggplot2_3.3.1 digest_0.6.25 foreach_1.5.0
## [13] htmltools_0.4.0 GO.db_3.11.4 gdata_2.18.0
## [16] magrittr_1.5 checkmate_2.0.0 memoise_1.1.0
## [19] cluster_2.1.0 doParallel_1.0.15 readr_1.3.1
## [22] fastcluster_1.1.25 annotate_1.67.0 prettyunits_1.1.1
## [25] jpeg_0.1-8.1 colorspace_1.4-1 blob_1.2.1
## [28] xfun_0.14 dplyr_1.0.0 crayon_1.3.4
## [31] RCurl_1.98-1.2 jsonlite_1.6.1 genefilter_1.71.0
## [34] impute_1.63.0 survival_3.1-12 iterators_1.0.12
## [37] glue_1.4.1 gtable_0.3.0 zlibbioc_1.35.0
## [40] XVector_0.29.3 scales_1.1.1 DBI_1.1.0
## [43] edgeR_3.31.1 Rcpp_1.0.4.6 viridisLite_0.3.0
## [46] xtable_1.8-4 progress_1.2.2 htmlTable_1.13.3
## [49] gridGraphics_0.5-0 flashClust_1.01-2 foreign_0.8-80
## [52] bit_1.1-15.2 preprocessCore_1.51.0 Formula_1.2-3
## [55] rsvg_2.1 htmlwidgets_1.5.1 httr_1.4.1
## [58] gplots_3.0.3 RColorBrewer_1.1-2 acepack_1.4.1
## [61] ellipsis_0.3.1 farver_2.0.3 pkgconfig_2.0.3
## [64] XML_3.99-0.3 nnet_7.3-14 locfit_1.5-9.4
## [67] dynamicTreeCut_1.63-1 labeling_0.3 ggplotify_0.0.5
## [70] tidyselect_1.1.0 rlang_0.4.6 later_1.0.0
## [73] AnnotationDbi_1.51.0 visNetwork_2.0.9 munsell_0.5.0
## [76] tools_4.1.0 generics_0.0.2 RSQLite_2.2.0
## [79] fastmap_1.0.1 evaluate_0.14 stringr_1.4.0
## [82] ggdendro_0.1-20 yaml_2.2.1 bit64_0.9-7
## [85] caTools_1.18.0 purrr_0.3.4 mime_0.9
## [88] compiler_4.1.0 rstudioapi_0.11 curl_4.3
## [91] plotly_4.9.2.1 png_0.1-7 tibble_3.0.1
## [94] geneplotter_1.67.0 stringi_1.4.6 highr_0.8
## [97] lattice_0.20-41 vctrs_0.3.0 pillar_1.4.4
## [100] lifecycle_0.2.0 BiocManager_1.30.10 data.table_1.12.8
## [103] bitops_1.0-6 grImport_0.9-3 httpuv_1.5.3.1
## [106] R6_2.4.1 latticeExtra_0.6-29 promises_1.1.0
## [109] bookdown_0.19 KernSmooth_2.23-17 gridExtra_2.3
## [112] codetools_0.2-16 MASS_7.3-51.6 gtools_3.8.2
## [115] DESeq2_1.29.4 GenomeInfoDbData_1.2.3 hms_0.5.3
## [118] grid_4.1.0 rpart_4.1-15 tidyr_1.1.0
## [121] rmarkdown_2.2 rvcheck_0.1.8 shiny_1.4.0.2
## [124] WGCNA_1.69 base64enc_0.1-3
This project has been funded by NSF awards: PGRP-1546879, PGRP-1810468, PGRP-1936492.
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